Plasmid

Part:BBa_K4895203:Design

Designed by: Patrick Jiang, Rori Hoover   Group: iGEM23_ASU   (2023-10-12)


mScarlet Optogentically controlled plasmid backbone (No His Tags)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 683
    Illegal NgoMIV site found at 815
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 1202
    Illegal NgoMIV site found at 1696
    Illegal NgoMIV site found at 1714
    Illegal NgoMIV site found at 1804
    Illegal AgeI site found at 1034
    Illegal AgeI site found at 2162
    Illegal AgeI site found at 3946
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Below is a review of the major design considerations

  1. Lac operon promoter and Lac operon terminator to ensure expression of mScarlet placed inside the Bbs1 cut sites
  2. T7 promoter, T7 terminator and T7 tag placed outside of the Bbs1 cut sites
  3. Kanamycin resistance
  4. FixLJ/K2 - non-coding regions that enforce optogenetic control of the plasmid



Source

This part contains portions of genomic sequences. The insert mScarlet, as well as Kanamycin resistance is not native to e.coli. Furthermore, the optogenetic control of the part is not native to e.coli either.

References